特性

应用

Strep-（WSHPQFEK）标签蛋白的高特异性亲和纯化

特异性

与Strep-tagged (WSHPQFEK) 蛋白亲和

结合载量

>7 mg/ml

配体

StrepTactin^®

粒径

30 μm

基材

6% 交联琼脂糖

充柱量

原包装含5%保存缓冲液

化学稳定性

2% Tween 20, 2% Triton X-100, 2% IGEPAL® 630/Nonidet P40, n-Octyl-beta-D-glucopyranoside, 0,2% n-Nonyl-beta-D-gluyopyranoside, 0,35% N-Decyl-beta-D-maltoside, 2% Lauryl-sacrosine, 0,1% SDS, 0,3% CHAPS, 1M Guanidine HCl, 1mM PMSF, 10% Ethanol, 5M NaCl, 2M (NH4)SO4, 1M CaCl2, 25% Glycerol

短期保存

中性缓冲液（详见操作指南Protocol）

长期保存

4°C，20%乙醇

PURIFIED PROTEIN

YEAR

AUTHOR

NPTX1

2020

Sticco M.J., Palomino P.A.P., Lukacsovich D., Thompson B.L., Földy C., Ressl S., Martinelli D.C.

Sarm1

2021

Li Y., Pazyra-Murphy M.F., Avizonis D., de Sá Tavares Russo M., Tang S., Chen C-Y., Hsueh Y-P., Bergholz S.J., Jiang T., Zhao J. J:, Zhu J., Woo Ko K., Milbrandt J., DiAntonio A., Segal R.A.

NMNAT

2022

Dingwall C.B ., Strickland A., Yum S.W., Yim A.K., Zhu J., Wang P.L., Yamada Y., Schmidt R.E, Sasaki Y., Bloom A.J., DiAntonio A., Milbrandt J.

TIR domains and TIR domain-encoding proteins

2022

Weagley J.S., Zaydman M., Venkatesh S., Sasaki Y., Damaraju N., Yenkin A., Buchser W., Rodionov D.M. Osterman A., Ahmed T., Barratt M.J., DiAntonio A., Milbrandt J., Gordon J.I.