特性

应用

Strep-（WSHPQFEK）标签蛋白的高特异性亲和纯化

特异性

与Strep-tagged (WSHPQFEK) 蛋白亲和

结合载量

>5 mg/ml

配基

StrepTactin®

粒径大小

100 μm

基材

6% cross-linked agarose

建议流速

0.25 - 1 ml/min

最大流速

6 ml/min

装柱量

原包装含50%保存缓冲液

化学稳定性

2% Tween 20, 2% Triton X-100, 2% IGEPAL® 630/Nonidet P40, n-Octyl-beta-D-glucopyranoside, 0,2% n-Nonyl-beta-D-gluyopyranoside, 0,35% N-Decyl-beta-D-maltoside, 2% Lauryl-sacrosine, 0,1% SDS, 0,3% CHAPS, 1M Guanidine HCl, 1mM PMSF, 10% Ethanol, 5M NaCl, 2M (NH4)SO4, 1M CaCl2, 25% Glycerol

短期储存

4°C，中性缓冲液

长期储存

4°C，中性缓冲液加20%乙醇

所需设备    

· Lysis Buffer

· Wash Buffer

· Elution Buffer

· Ice bath

· Refrigerated centrifuge for 50 ml tube (min 10,000 x g)

· 50 mL centrifuge tube

· Micropipettor and Micropipetting tips

· Disposable gravity flow columns with capped bottom outlet, 2 ml

· pH meter

· End-over-end shaker

· SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment