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PureCube 100 Co-NTA Agarose
The term Co-NTA refers to a copper Ion that has been coupled Nitrilotriacetic acid. Co-NTA can then be coupled to agarose or magnetic beads for IMAC (Immobilized Metal Chelate Affinity Chromatography). This is a purification method to obtain functional His-tagged protein. Cube Biotech offers multiple different Co-NTA based products for protein purification. Our PureCube 100 Co-NTA agarose consist of agarose resin beads with a diameter of 100 µm. They are used for the purification of active & stable His Tag protein from e.g. cell lysates. Our agarose resins are suited for batch spin columns and FPLC. Co-NTA provides purer protein eluates that Ni-NTA. Furthermore we recommend our PureCube Co-NTA resin with 40 µm diameter and for cases that require a drastically increased flow rate of the beads our Purecube XL Co-NTA agarose beads.
Our agarose resins are suited for batch spin columns and FPLC. Co-NTA provides purer protein eluates that Ni-NTA.
1. Protein Binding Capacity
The protein binding capacity is up to 30 mg/mL, as determined by purification of 6xHis-tagged GFP protein from E.coli cleared lysates, and quantified via spectrophotometry. Proteins are eluted with high purity.
2. Compatibility
PureCube 100 Co-NTA Agarose is very stable and can resist the following conditions in most situations: pH 3-13, 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile, 10 mM DTT, 1 mM EDTA, 1 M NaOH.
Type: | Agarose |
Ligand: | NTA |
Coupled Ion: | Co2+ |
Usage | Specific binding and purification of 6x His-tagged proteins |
Specifity | Affinity to His-tagged proteins |
Binding capacity | >30 mg/mL |
Chelator stability | Stable in buffer containing 10 mM DTT and 1 mM EDTA |
Filling quantity | Delivered as a 50 % suspension |
Bead size | 40 μm |
Bead Ligand | Co-NTA |
Required equipment |
· Lysis Buffer · Wash Buffer · Elution Buffer · Ice bath · Refrigerated centrifuge for 50 mL tube (min 10,000 x g) · 50 mL centrifuge tube · Micropipettor and Micropipetting tips · Disposable gravity flow columns with capped bottom outlet, 2 ml · pH meter · End-over-end shaker · SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment |
货号 | 产品 | 粒径 （μm） | 结合 载量 | 运输 温度 | 储存 温度 | 应用 |
74401 | PureCube 100 Co-NTA Agarose(1ml) | 100 | 30mg/mL | 室温 | 4° C | 100 系列适用于高流速要求的His标签重组蛋白的纯 化。流速高达1000cm/h最 大压力高达300kPa，pH稳定工作范围3-13，在10mM DTT，1mM EDTA，1M NaOH 环境保持稳定，相较于 100Ni-NTA，Co-NTA有更高特异 |
74403 | PureCube 100 Co-NTA Agarose(10ml) | 100 | 30mg/mL | 室温 | 4° C | |
74405 | PureCube 100 Co-NTA Agarose(50ml) | 100 | 30mg/mL | 室温 | 4° C | |
74410 | PureCube 100 Co-NTA Agarose(250ml) | 100 | 30mg/mL | 室温 | 4° C | |
74412 | PureCube 100 Co-NTA Agarose(500ml) | 100 | 30mg/mL | 室温 | 4° C | |
74415 | PureCube 100 Co-NTA Agarose(1000ml) | 100 | 30mg/mL | 室温 | 4° C | |
74420 | PureCube 100 Co-NTA Agarose(5000ml) | 100 | 30mg/mL | 室温 | 4° C |
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