31101 ；31103 ；31105 ；31110 ；31112 ；31115 ；31120；cube；德国库柏；Ni-NTA；Cube Biotech；Agarose；琼脂糖；蛋白纯化；蛋白纯化试剂

PureCube Ni-NTA Agarose

产品简介

产品优势

产品详情

产品参数

PureCube Ni-NTA Agarose

PureCube His Affinity Agarose (NTA)是针对组氨酸标签的亲和层析产品，组氨酸标签是应用广泛的亲和层析标签，这种标签具有小尺寸、低免疫原性的特点和在多种天然或变性条件下的广泛适用性，NTA相对于IDA具有更高的洗脱液纯度。

产品优势

(1)、德国制造，品质与信誉的坚实保障；

(2)、结合载量可高达80mg/mL，目前市面上非常高的结合能力；
(3)、更优越的DTT和EDTA稳定性和耐受性；
(4)、凝胶的多孔性和均匀粒径，使蛋白与凝胶之间有很好地相互作用；
(5)、适合低压环境下的纯化过程（大规模纯化制备或预装柱均可使用）；
(6)、为研发、生产降低成本；
(7)、适用于大肠杆菌和真核细胞的裂解液和上清液；
(8)、同时可装载Co, Cu, Zn和其它金属离子。

产品详情

1. 收率高，纯度高

Fig. 1: Over 20% more yield obtained with PureCube Ni-NTA Agarose. SDS-PAGE of GFP expressed in E. coli and purified in gravity columns with PureCube Ni-NTA Agarose and Ni-NTA resin from Competitor Q. 80 mg/mL protein yield was obtained with PureCube Ni-NTA Agarose (E1–E4, Cube) compared to 65 and 48 mg/mL, respectively, with the widely used alternative resins G and Q (E1–E4, Competitor G / Competitor Q).

	Cube Biotech
蛋白结合能力	≤80mg/mL

2. 优越的DTT和EDTA稳定性

Fig. 2: NTA is robust in the presence of reducing and chelating agents. GFP-His was purified on gravity columns containing PureCube Ni-NTA Agarose after exposing the resin for 1 h to 3 concentrations of DTT or EDTA. NTA exhibits a shallow decay rate in binding capacity.

PureCube Ni-NTA琼脂糖在DTT和EDTA存在下非常坚实，在稳定性测试中，将PureCube Ni-NTA琼脂糖置于DTT与EDTA浓度逐渐升高的环境中处理1小时。再用处理过的凝胶在重力层析柱中纯化大肠杆菌表达的GFP-His，凝胶的蛋白结合能力因存在DTT与EDTA而下降，但是衰减率平缓，即使在1.5 mM EDTA，凝胶依然具有其结合能力的54%。

	Cube Biotech
DTT稳定性	<10mM
EDTA稳定性	<1.5mM

产品参数

应用	Specific binding and purification of 6x his-tagged proteins
特异性	Affinity to His-tagged proteins
结合能力	>80 mg/mL
Bead Ligand	Ni-NTA
粒径大小	40 μm
蝥合剂稳定性	Stable in buffer containing 10 mM DTT and 1 mM EDTA
保存	Delivered as a 50 % suspension
所需试剂耗材	Lysis Buffer Wash Buffer Elution Buffer Ice bath Refrigerated centrifuge for 50 mL tube (min 10,000 x g) 50 mL centrifuge tube Micropipettor and Micropipetting tips Disposable gravity flow columns with capped bottom outlet, 2 ml pH meter End-over-end shaker SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment

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货号	产品	粒径（μm）	结合载量	运输温度	储存温度	应用
31101	PureCube Ni-NTA Agarose(1ml)	40	80mg/mL	室温	4° C	适用于 His 标签重组蛋白的纯化 , 超高蛋白结合载量。pH 稳定工作范围2-14, 最大流速为6mL/min。在10mM DTT 和1mM EDTA的环境下保持稳定
31103	PureCube Ni-NTA Agarose(10ml)	40	80mg/mL	室温	4° C
31105	PureCube Ni-NTA Agarose(50ml)	40	80mg/mL	室温	4° C
31110	PureCube Ni-NTA Agarose(250ml)	40	80mg/mL	室温	4° C
31112	PureCube Ni-NTA Agarose(500ml)	40	80mg/mL	室温	4° C
31115	PureCube Ni-NTA Agarose(1000ml)	40	80mg/mL	室温	4° C
31120	PureCube Ni-NTA Agarose(5000ml)	40	80mg/mL	室温	4° C

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HIS标签

NI-NTA

Co-NTA

INDIGO

NTA XL

Ni-NTA XL

Co-NTA XL

100 NTA

100 Ni-NTA

100 Co-NTA

GST标签

Glutathione

Glutathione Agarose XL

GST相关产品

Rho1D4标签

Rho1D4

Rho1D4相关产品

Nanodisc（纳米磷脂盘）

MSP Proteins

7-8nm

9-10nm

12-14nm

17nm

DIBMA

SMALP

AASTY

Ultrasolute™ Amphipol

Synthetic Screening Kit

膜蛋白结晶板

MO Plates

Strep Tactin

HiCap StrepTactin

ABT

排阻层析（分子筛）

低耐压

高耐压

低耐压预装柱

离子交换

高耐压

亲和层析

组氨酸标签(His-Tag)

抗体纯化

生物素/亲和素纯化

GST标签（谷胱甘肽）

亲和偶联

氨基

羧基

磁性琼脂糖

抗体纯化

蛋白纯化

AppliChrom

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PureCube Ni-NTA Agarose

A Novel Glutamyl (Aspartyl)-Specific Aminopeptidase A from Lactobacillus delbrueckii with Promising Properties for Application

Protein–Polymer Microcapsules for PCR Technology

Structural insights into ion conduction by channelrhodopsin 2

A New Homo-Hexamer Mn-Containing Catalase from Geobacillus sp. WCH70

Cell-free production of membrane proteins in Escherichia coli lysates for functional and structural studies

A novel glutamyl (aspartyl)-specific aminopeptidase A from Lactobacillus delbrueckii with promising properties for application

Molecular Characterization of Ethylene Response Sensor 1 (BoERS1) in Bambusa oldhamii

A natural variant of arylsulfatase from Kluyveromyces lactis shows no formylglycine modification and has no enzyme activity

Influence of the metal ion on the enzyme activity and kinetics of PepA from Lactobacillus delbrueckii.

Buß, Maren et al.(2019). Specific high affinity interaction of Helicobacter pylori CagL with integrin α V β 6 promotes type IV secretion of CagA into human cells. The FEBS Journal. 10.1111/febs.14962.

The SnRK1-eIFiso4G1 signaling relay regulates the translation of specific mRNAs in Arabidopsis under submergence

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