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PureCube Co-NTA MagBeads XL
The term Co-NTA refers to a copper Ion that has been coupled Nitrilotriacetic acid. Co-NTA can then be coupled to agarose or magnetic beads for IMAC (Immobilized Metal Chelate Affinity Chromatography). This is a purification method to obtain functional His-tagged protein. Cube Biotech offers multiple different Co-NTA based products for protein purification. In special cases normal sized magnetic beads are not sufficient. Therefore we offer PureCube Co-NTA MagBeads Xl sized. For our regular sized Co-NTA MagBeads (~30 µm) refer to this page. Of course we are also selling our PureCube MagBead separator for the magnetic separation in case you do not have one yet.
1. Protein Binding Capacity
The protein binding capacity is ~20 mg protein per mL of settled beads, as determined by purification of 6xHis-tagged GFP protein from E.coli cleared lysates, and quantified via spectrophotometry.
2. Compatibility
PureCube Co-NTA MagBeads XL are very stable and can resist the following conditions in most situations: pH 2-14, 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/ v) acetonitrile.
Type: | Magnetic Beads |
Ligand: | NTA |
Coupled Ion: | Co2+ |
Usage | Specific binding and purification of 6x his-tagged proteins |
Specifity | Affinity to His-tagged proteins |
Binding capacity | >20 mg/mL |
Bead Ligand | Co-NTA |
Bead size | 90 µm |
Filling quantity | Delivered as a 25 % suspension |
Chelator stability | Stable in buffer containing 10 mM DTT and 1 mM EDTA |
pH stability | 2-14 |
Other stabilities | 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile |
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