FEATURE

MSP1E3D1-His, lyophilized protein

Nanodisc Assembly Kit MSP1E3D1-His dmpc/POPC

Available origins

Human, rat, mouse,

Purity

> 90% (Determined by SDS-PAGE)

Number of amino acids

· 277 (Human origin)

· 276 (Mouse origin)

· 267 (Rat origin)

277

Molecular Mass

· 32,600 Da (Human origin)

· 22,534 Da (Mouse origin)

· 31,572.76 Da (Rat origin)

32,600 Da

Extinction coefficient (in water) ε280

· 29,400 M^-1cm^-1 (Human origin)

· 32,430 M^-1cm^-1 (Mouse origin)

· 36,440 M^-1cm^-1 (Rat origin)41,940 M^-1cm^-1 (Alpaca origin)

29,400 M^-1cm^-1

Buffer

20 mM TRIS pH 7.4; 100 mM NaCl; 0.5 mM EDTA

Sequence (Human origin), for the other origins please send request

GHHHHHHH DYDIPTT ENLYFQG STFSKLREQLG PVTQEFWDNLEKETEGLRQEMS KDLEEVKAKVQ PYLDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEKLS PLGEEMRDRARAHVDALRTHLA PYLDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEKLS PLGEEMRDRARAHVDALRTHLA PYSDELRQRLAARLEALKENGG ARLAEYHAKATEHLSTLSEKAK PALEDLRQGLL PVLESFKVSFLSALEEYTKKLNTQ

Affinity tag

N-Terminal His-tag

Shipping Temperature

Room Temperature

Storage of lyophilized protein

-20°C for several months

Storage of reconstituted protein

2-8°C for several days

Helpful literature references

1. Bayburt, T.H. et al. Reconstitution and imaging of a membrane protein in a nanometer-size phospholipid bilayer. J. Struct. Biol. (1998), 123(1):37-44

2. Civjan, N.R. et al. Direct solubilization of heterologously expressed membrane proteins by incorporation into nanoscale lipid bilayers. BioTechniques (2003) 35:556-563

3. Hagn, F. et al. Optimized phospholipid bilayer nanodiscs facilitate high-resolution structure determination of membrane proteins. J.Am.Chem. Soc. (2013), 135:1919-1925

4. Proverbio D., et al. Functional properties of cell-free expressed human endothelin A and endothelin B receptors in artifical membrane environments. Biochim.Biophys. Acta (2013), 1828(9):2182-92

5. Roos, C., et al. 2014, High-level Cell-free production of membrane proteins with Nanodiscs. In: Alexandrov, K., and Johnston W.A. (eds) Cell-free protein synthesis: Methods and Protocols. Methods in Molecular Biology, vol. 1118, Springer Science+Business Media.

FEATURE - PHOSPHOLIPIDS

CAS-Number

· DMPC: 18194-24-6

· POPC: 26853-31-6

Molecular Mass

· DMPC: 677.93 Da

· POPC: 760.08 Da

Chemical Formula

· DMPC: C₃₆H₇₂NO₈P

· POPC: C₄₂H₈₂NO₈P