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PureCube Glyco-DIBMA
Glyco-DIBMA is a modified version of DIBMA with an added sugar side-chain to the polymer molecule. Its purpose is to enhance DIBMA's range for solubilizing membrane proteins.
DIBMA / Nanodisc Screening: Each membrane protein may perform better or differently with certain synthetic polymers. This is due to the characteristic composition of phospholipids surrounding the membrane proteins. Therefore a screening process for the correct DIBMA/Polymer is required in before. For this purpose, we offer our synthetic nanodisc screening kit MAXI which contains DIBMA, AASTY, and SMA.
For the longest time, the science behind membrane proteins relied on detergents for both solubilization and stabilization. However, detergents such as DDM or LMNG come with their set of problems. A time-consuming screening process for the correct detergent and the constant need to add it to all buffers can be avoided. But this applies to all synthetic nanodiscs.
Figure 1 shows the amount of membrane protein of interest that was stabilized by a DIBMALP in comparison to a construct using the detergent DDM. As it can clearly be seen DDM has way fewer specific bands at the desired kDA values of around 40-60 kDa.
Fig. 2: DIBMA precipitation in relation to the ion concentration. The shown concentrations start at 5 mM and are increasing in 5 mM steps up to 50 mM.
FEATURES | |
State | Lyophilized Powder, |
Molecular weight | 15701 g/Mol |
pH after solving | 7.5 |
Solubility | >10% |
Divalent cationic tolerance | < 4 mM |
Adsorbance (280 nm, 1% solution) | > 0.3 |
Buffer | HEPES |
Specific gravity | 1.1 |
Storage | 4°C for long-term |
Structure |  |
Order number | Products | Shipment Temperature | Storage Temperature |
18411 | Glyco-DIBMA, 10x50mg | Ambient temperature | -20°C |
18412 | Glyco-DIBMA, 1g | Ambient temperature | -20°C |
18413 | Glyco-DIBMA, 10x1g | Ambient temperature | -20°C |
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