FEATURE

MSP2N2-His, lyophilized protein

Nanodisc Assembly Kit MSP1E3D1-His dmpc/POPC

Available origins

Human, mouse

Human

Purity

> 90% (Determined by SDS-PAGE)

Number of amino acids

· 392 (Human origin)

· 388 (mouse origin)

392

Molecular Mass

· 45,670 Da (Human origin)

· 45,526 Da (Mouse origin)

45,670 Da

Extinction coefficient (in water) ε280

· 44,920 M^-1cm^-1 (Human origin)

· 39,880 M^-1cm^-1 (Mouse origin)

44,920 M^-1cm^-1

Buffer

20 mM TRIS pH 7.4; 100 mM NaCl; 0.5 mM EDTA

Sequence (Human origin), for the other origins please send request

GHHHHHHH DYDIPTT ENLYFQG STFSKLREQLG PVTQEFWDNLEKETEGLRQEMS KDLEEVKAKVQ PYLDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEKLS PLGEEMRDRARAHVDALRTHLA PYSDELRQRLAARLEALKENGG ARLAEYHAKATEHLSTLSEKAK PALEDLRQGLL PVLESFKVSFLSALEEYTKKLNTQ GT PVTQEFWDNLEKETEGLRQEMS KDLEEVKAKVQ PYLDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEKLS PLGEEMRDRARAHVDALRTHLA PYSDELRQRLAARLEALKENGG ARLAEYHAKATEHLSTLSEKAK PALEDLRQGLL PVLESFKVSFLSALEEYTKKLNTQ

Affinity tag

N-Terminal His-tag

Shipping Temperature

Room Temperature

Storage of lyophilized protein

-20°C for several months

Storage of reconstituted protein

2-8°C for several days

Helpful literature references

1. Bayburt, T.H. et al. Reconstitution and imaging of a membrane protein in a nanometer-size phospholipid bilayer. J. Struct. Biol. (1998), 123(1):37-44

2. Civjan, N.R. et al. Direct solubilization of heterologously expressed membrane proteins by incorporation into nanoscale lipid bilayers. BioTechniques (2003) 35:556-563

3. Hagn, F. et al. Optimized phospholipid bilayer nanodiscs facilitate high-resolution structure determination of membrane proteins. J.Am.Chem. Soc. (2013), 135:1919-1925

4. Proverbio D., et al. Functional properties of cell-free expressed human endothelin A and endothelin B receptors in artifical membrane environments. Biochim.Biophys. Acta (2013), 1828(9):2182-92

5. Roos, C., et al. 2014, High-level Cell-free production of membrane proteins with Nanodiscs. In: Alexandrov, K., and Johnston W.A. (eds) Cell-free protein synthesis: Methods and Protocols. Methods in Molecular Biology, vol. 1118, Springer Science+Business Media.

FEATURE - PHOSPHOLIPIDS

CAS-Number

· DMPC: 18194-24-6

· POPC: 26853-31-6

Molecular Mass

· DMPC: 677.93 Da

· POPC: 760.08 Da

Chemical Formula

· DMPC: C₃₆H₇₂NO₈P

· POPC: C₄₂H₈₂NO₈P