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SMALP 140
The synthethic polymer SMALP 140, among our other synthetic nanodisc products has been developed for for the solubilization & subsequent purification of membrane proteins from all kinds of cell membranes. SMALP products do this by using the phospholipids around the membrane proteins and form them into a disc-shaped structure in which the membrane proteins are centered. SMALP 140 + its corresponding buffer are shipped in a lyophilized state, so they have to be dissolved in dH2O. Then the product is ready-to-use. SMALP 140 has a amber color in solution. We recommend using affinity chromatography for the follow-up purification of the membrane protein of interest. For membrane protein purification we recommed to use the Rho1D4-tag. Cube Biotech offers the matching products for this purpose.
SMALP Screening: Different membrane protein usually perform different under different SMAs variants respectively. To ease the resulting screening process we compiled all of our SMAs into a convenient SMALP Screening Set. Furthermore if you like to compare proteins's performance between SMA and DIBMA in synthetic nanodiscs, we also offer our, that includes both our SMA and DIBMA products.
Figure 1: Western Blot of a purified membrane protein after stabilization through SMA. Reproduced with permission from Ms S. Nestorow, University of Birmingham.
FEATURE | |
State | Lyophilized Powder, to be solved with water |
Polymer styrene-to-maleic anhydride ratio | 1.4:1 |
Molecular weight | 5,000 Da |
pH after solving | 7.5 |
Solubility | 45 % |
Divalent cationic tolerance | < 5 mM |
Color | Amber |
Odor | Odorless |
Freeze-thaw stable? | Yes |
Storage | 4°C for long-term |
Structure |  |
Order number | Products | Shipment Temperature | Storage Temperature |
18220 | SMALP 140 10x50 mg | Ambient temperature | -20°C |
18221 | SMALP 140 1 g | Ambient temperature | -20°C |
18222 | SMALP 140 10 g | Ambient temperature | -20°C |
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Noh I., Guo Z., Zhou J., Gao W., Fang R.H., Zhang L.
Univerzita Karlova Přírodovědecká fakulta.
Zhou R., Zhang S., Nguyen H.T., Ding H., Gaffney A., Kappes J.C.,Smith Ill A.B.S, Sodroski J.G.
bioRxiv.