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AASTY 系列产品（1）
AASTY系列产品（1）指AASTY 6-45、AASTY 6-50、AASTY 6-55等产品。
The exact composition of AASTY copolymers shows different extraction efficiency, depending on the lipid composition of the lipid bilayers being formulated into nanodiscs. As AASTY is made by controlled radical polymerization techniques, the dispersity of polymer molecular weight distribution is low, and the molecular weights are controlled. This means that excess AASTY copolymer can be removed by dialysis after nanodisc formation. Based on previous findings on SMA, it is the expectation that AASTY of different molecular weights will display different rates of nanodisc formation, extraction efficacy, and stability of resulting nanodiscs.
Every membrane protein solubilization needs to undergo a screening process in before. The characteristic phospholipid environment surrounding the different membrane proteins in question performs differently well with each polymer. To support you in this process we offer a handy Screening Kit for AASTYs to test them all. Additionally, we recommend the two following publications if you would like to get further information: Smith et al. 2020 & Timcenko et al. 2022.
As every membrane protein, surrounded by its characteristic phospholipid environment, performs differently well with every other polymer, it is generally advisable to screen for the most viable conditions of your protein of interest. However, when you're interested in conditions with lower polymer concentrations, AASTYs are a good choice to start with your experiments.
FEATURE | AASTY 6-45 | AASTY 6-50 | AASTY 6-55 |
State | Lyophilized Powder,to be solved with water | ||
Molecular Weight | 5-6 kDa | ||
Buffer | HEPES | ||
pH after solving | 7.5 | ||
Divalent cationic tolerance | 6 mM | ||
Acrylic Acid to Styrene Ratio | 45% to 55% | 50% to 50% | 55% to 45% |
Storage | 4°C for long-term | ||
Structure |  | ||
Order number | Products | Shipment Temperature | Storage Temperature |
18250 | AASTY 6-45, 10x50mg | Ambient temperature | -20°C |
18251 | AASTY 6-45, 1g | Ambient temperature | -20°C |
18252 | AASTY 6-45, 10x1g | Ambient temperature | -20°C |
18260 | AASTY 6-50, 10x50mg | Ambient temperature | -20°C |
18261 | AASTY 6-50, 1g | Ambient temperature | -20°C |
18262 | AASTY 6-50, 10x1g | Ambient temperature | -20°C |
18270 | AASTY 6-55, 10x50mg | Ambient temperature | -20°C |
18271 | AASTY 6-55, 1g | Ambient temperature | -20°C |
18272 | AASTY 6-55, 10x1g | Ambient temperature | -20°C |
18285 | AASTY Screening Kit (6x2x50 mg) | Ambient temperature | -20°C |
18295 | Synthetic Nanodisc Screening Kit MAXI (17x2x50 mg) | Ambient temperature | -20°C |
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