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PureCube Nanodisc MSP（7-8nm，His标签）系列
PureCube Nanodisc MSP（7-8nm，His标签）系列包含以下产品：Nanodisc Assembly Kit MSP1D1 dH5-His POPC、Nanodisc Assembly Kit MSP1D1 dH5-His DMPC、MSP1D1 dH5-His, lyophilized protein.
This product is a MSP nanodisc assembly kit. It contains a vial of lyophilized membrane scaffold protein MSP1D1 dh5 (His-tagged) and a vial of DMPC or POPC phopshospholipid to assemble a MSP nanodisc.
With this assembly kit both cellular as well as cell-free expressed proteins can be stabilized in MSP nanodiscs. Just use the corresponding protocol for each purpose (see below). The MSP protein contains a His-tag, keep that in mind when choosing the affinity tag for your membrane protein.
Figure 1: Schematic of a MSP nanodisc. A Membrane-scaffold-protein (MSP, green) forms a disc-shaped structure with some phospholipids.
FEATURE - MSP PROTEIN | |
Purity | > 90% (Determined by SDS-PAGE) |
Number of amino acids | 184 |
Molecular Mass | 21,468 Da |
Extinction coefficient (in water) ε280 | 19,940 M-1cm-1 |
Buffer | 20 mM TRIS pH 7.4; 100 mM NaCl; 0.5 mM EDTA |
Origin | Human |
Sequence (1-Letter Code), for more details, see datasheet | MGSSHHHHHH ENLYFQ GSTFSKLREQ LGPVTQEFWD NLEKETEGLR QEMSKDLEEV AKVQPYLDDF QKKWQEEMEL RQKVEPLGEE MRDRARAHVD ALRTHLAPYS DELRQRLAAR LEALKENGGA RLAEYHAKAT EHLSTLSEKA KPALEDLRQG LLPVLESFKV SFLSALEEYT KKLNTQ |
Affinity tag | N-Terminal His-tag |
Shipping Temperature | Room Temperature |
Storage of lyophilized protein | -20°C for several months |
Storage of reconstituted protein | 2-8°C for several days |
FEATURE - PHOSPHOLIPIDS CAS-Number · DMPC: 18194-24-6 · POPC: 26853-31-6 Molecular Mass · DMPC: 677.93 Da · POPC: 760.08 Da Chemical Formula · DMPC: C36H72NO8P · POPC: C42H82NO8P
Order number | Products | Shipment Temperature | Storage Temperature |
26221 | Nanodisc Assembly Kit MSP1D1 dH5-His_DMPC | Ambient temperature | -20°C |
26223 | Nanodisc Assembly Kit MSP1D1 dH5-His_POPC | Ambient temperature | -20°C |
26122 | MSP1D1 dH5-His, lyophilized protein (2 mg) | Ambient temperature | -20°C |
26126 | MSP1D1 dH5-His, lyophilized protein (10 mg) | Ambient temperature | -20°C |
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