FEATURE

Available origins

Human, mouse

Purity

> 90% (Determined by SDS-PAGE)

Number of amino acids

· 256 (Human origin)

· 254 (Mouse origin)

Molecular Mass

· 29,982 Da (Human origin)

· 29,786 (Mouse origin)

Extinction coefficient (in water) ε280

· 26,930 M-1cm-1 (Human origin)

· 29,450 M-1cm-1 (Mouse origin)

Buffer

20 mM TRIS pH 7.4; 100 mM NaCl; 0.5 mM EDTA

Sequence (Human origin), for the other origins please send request

G STFSKLREQLG PVTQEFWDNLEKETEGLRQEMS KDLEEVKAKVQ PYLDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEKLS PLGEEMRDRARAHVDALRTHLA PYLDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEKLS PLGEEMRDRARAHVDALRTHLA PYSDELRQRLAARLEALKENGG ARLAEYHAKATEHLSTLSEKAK PALEDLRQGLL PVLESFKVSFLSALEEYTKKLNTQ

Affinity tag

none

Shipping Temperature

Room Temperature

Storage of lyophilized protein

-20°C for several months

Storage of reconstituted protein

2-8°C for several days

Helpful literature references

1. Bayburt, T.H. et al. Reconstitution and imaging of a membrane protein in a nanometer-size phospholipid bilayer. J. Struct. Biol. (1998), 123(1):37-44

2. Civjan, N.R. et al. Direct solubilization of heterologously expressed membrane proteins by incorporation into nanoscale lipid bilayers. BioTechniques (2003) 35:556-563

3. Hagn, F. et al. Optimized phospholipid bilayer nanodiscs facilitate high-resolution structure determination of membrane proteins. J.Am.Chem. Soc. (2013), 135:1919-1925