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SMALP 300
The SMALP products have been developed for biomedical purposes, in particular, for the solubilization of (biological) lipid membranes and isolation & purification of integral membrane proteins. After solving the SMA + buffer powder in water, the product is ready-to-use. It can primarily used for all cell membrane hosts, including bacteria, yeast and human cells. After successfull solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommed to use the Rho1D4-tag. Cube Biotech offers the matching products for this purpose.
SMALP Screening: Different membrane protein usually perform different under different SMAs variants respectively. To ease the resulting screening process we compiled all of our SMAs into a convenient SMALP Screening Set. Furthermore if you like to compare proteins's performance between SMA and DIBMA in synthetic nanodiscs, we also offer our, that includes both our SMA and DIBMA products.
Figure 1: Western Blot of a purified membrane protein after stabilization through SMA. Reproduced with permission from Ms S. Nestorow, University of Birmingham.
FEATURE | |
State | Lyophilized Powder, to be solved with water |
Polymer styrene-to-maleic anhydride ratio | 3:1 |
Molecular weight | 10,000 Da |
pH after solving | 7.5 |
Solubility | 35 % |
Divalent cationic tolerance | < 5 mM |
Color | Colorless |
Odor | Odorless |
Freeze-thaw stable? | Yes |
Storage | 4°C for long-term |
Structure |  |
Order number | Products | Shipment Temperature | Storage Temperature |
18200 | SMALP 300 10x50 mg | Ambient temperature | -20°C |
18201 | SMALP 300 1 g | Ambient temperature | -20°C |
18202 | SMALP 300 10 g | Ambient temperature | -20°C |
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Noh I., Guo Z., Zhou J., Gao W., Fang R.H., Zhang L.
Univerzita Karlova Přírodovědecká fakulta.
Zhou R., Zhang S., Nguyen H.T., Ding H., Gaffney A., Kappes J.C.,Smith Ill A.B.S, Sodroski J.G.
bioRxiv.