FEATURE - MSP PROTEIN

Origin of MS Protein

Human

Purity

> 90% (Determined by SDS-PAGE)

Number of amino acids

· 201 (Human origin)

· 198 (Mouse origin)

Molecular Mass

· 23,329 Da (Human origin)

· 22.948 Da (Mouse origin)

Extinction coefficient (in water) ε280

· 25,440 M-1cm-1 (Human origin)

· 20,970 M-1cm-1 (Mouse origin)

Buffer

20 mM TRIS pH 7.4; 100 mM NaCl; 0.5 mM EDTA

Sequence (Human origin), for the other origins please send request

GLKLLDNWDSVTSTFSKLREQLG PVTQEFWDNLE KETEGLRQEMS KDLEEVKAKVQ PYLDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEKLS PLGEEMRDRARAHVDALRTHLA PYSDELRQRLAARLEALKENGG ARLAEYHAKATEHLSTLSEKAK PALEDLRQGLL PVLESFK VSFLSALEEY TKKLNTQ

Affinity tag

none

Shipping Temperature

Room Temperature

Storage of lyophilized protein

-20°C for several months

Storage of reconstituted protein

2-8°C for several days

Helpful literature references

1. Bayburt, T.H. et al. Reconstitution and imaging of a membrane protein in a nanometer-size phospholipid bilayer. J. Struct. Biol. (1998), 123(1):37-44

2. Civjan, N.R. et al. Direct solubilization of heterologously expressed membrane proteins by incorporation into nanoscale lipid bilayers. BioTechniques (2003) 35:556-563

3. Hagn, F. et al. Optimized phospholipid bilayer nanodiscs facilitate high-resolution structure determination of membrane proteins. J.Am.Chem. Soc. (2013), 135:1919-1925

4. Proverbio D., et al. Functional properties of cell-free expressed human endothelin A and endothelin B receptors in artifical membrane environments. Biochim.Biophys. Acta (2013), 1828(9):2182-92

5. Roos, C., et al. 2014, High-level Cell-free production of membrane proteins with Nanodiscs. In: Alexandrov, K., and Johnston W.A. (eds) Cell-free protein synthesis: Methods and Protocols. Methods in Molecular Biology, vol. 1118, Springer Science+Business Media.

FEATURE - PHOSPHOLIPIDS

CAS-Number

· DMPC: 18194-24-6

· POPC: 26853-31-6

Molecular Mass

· DMPC: 677.93 Da

· POPC: 760.08 Da

Chemical Formula

· DMPC: C₃₆H₇₂NO₈P

· POPC: C₄₂H₈₂NO₈P