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Synthetic Nanodisc Screening Kit MINI, (8x2x50 mg)
Finding the best performing synthetic nanodisc polymer for your respective nanodisc can be tricky. Especially if you have never worked with your current membrane protein of interest before. For these cases we at Cube Biotech compiled our most prominent SMALP and DIBMA products into this practical Synthetic Nanodisc Screening Set for your convenience!
All 8 products are buffered in HEPES to provide maximal comparability between the individual screening assays. Which 8 products are include in the Kit precisely can be found in the product's description.
Figure 1 shows the necessity for a SMALP screen instead of using a random SMALP product. Note that the detergent LMNG may provide the mighest protein yield, but it has to be added to the purified protein constantly and it might interfere with the SPIKE protein's function.
Included SMALP Products
Feature Polymer styrene-to-maleic-anhydride ratio [n:m] 1.4:1 1.4:1 2:1 3:1 Molecular weight 5,000 Da 5,000 Da 6,500 Da 10,000 Da Form Lyophilized powder Lyophilized powder Lyophilized powder Lyophilized powder pH, after solving 7.5 7.5 7.5 7.5 Divalent cationic tolerance < 5 mM < 100 mM < 5 mM < 5 mM Color Amber Brownish Yellowish Colorless Odor Odorless Odorless Odorless Odorless Other noteworthy feature is imidized with DMAPA Most commonly used SMA
Included DIBMA Products
Feature Formula Weight 10,000 Da 12,000 Da 10,000 Da 10,000 Da Solubility > 10 % in H2O Absorbance at 280 nm < 0.3 (1 % solution) Mg2+ Tolerance 25 mM 25 mM >50 mM >50 mM Ca2+ Tolerance starts to precipitate 50 mM starts to precipitate at 50 mM >50 mM >50 mM