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Synthetic Nanodisc Screening Kit MAXI (16x2x50 mg)
Finding the best performing snythetic nanodisc polymer for your respective nanodisc can be tricky. Especially if you have never worked with your current membrane protein of interest before. For these cases we at Cube Biotech compiled our most prominent SMALP, DIBMA and AASTY products into this practical Synthetic Nanodisc Screening Set MAXI for your convenience!
All 16 products are buffered in HEPES to provide maximal comparability between the individual screening assays. Which 16 products are include in the Kit precisely can be found in the product's description.
Figure 1 shows the necessity for a screening assay instead of using a random polymer product.
Figure 1: Performance of membrane protein solubilization under different synthetic polymers. All samples contained the same membrane protein of interest. Furthermore, two different polymer concentrations were tested as well. 2.5% and 0.5%.
Included SMALP Products
Feature Polymer styrene-to-maleic-anhydride ratio [n:m] 1.4:1 1.4:1 2:1 3:1 Molecular weight 5,000 Da 5,000 Da 6,500 Da 10,000 Da Form Lyophilized powder Lyophilized powder Lyophilized powder Lyophilized powder pH, after solving 7.5 7.5 7.5 7.5 Divalent cationic tolerance < 5 mM < 100 mM < 5 mM < 5 mM Color Amber Brownish Yellowish Colorless Odor Odorless Odorless Odorless Odorless Other noteworthy feature is imidized with DMAPA Most commonly used SMA
Included DIBMA Products
Feature Formula Weight 10,000 Da 12,000 Da 10,000 Da 10,000 Da 15700 Da Solubility > 10 % in H2O Absorbance at 280 nm < 0.3 (1 % solution) Mg2+ Tolerance 25 mM 25 mM >50 mM >50 mM 25 mM Ca2+ Tolerance starts to precipitate 50 mM starts to precipitate at 50 mM >50 mM >50 mM starts to precipitate at 50 mM
Included AASTY Products
Feature Molecular weigth 5-6 kDa 5-6 kDa 5-6 kDa 10-11 kDa 10-11 kDa 10-11 kDa pH after solving 7.5 7.5 7.5 7.5 7.5 7.5 Divalent cationic tolerance 6 mM 6 mM 6 mM 6 mM 6 mM 6 mM Acrylic Acid to Styrene Ratio 45% to 55% 50% to 50% 55% to 45% 45% to 55% 50% to 50% 55% to 45%
Ultrasolute™ Amphipol Products
Feature Ultrasolute™ 17 pH after solving 7.5 ± 0.3 7.5 ± 0.3 Solubility 10% (H2O) 10% (H2O) Molecules that make up the polymer 2-Cyclohexyl-ethyl acrylamide / acrylic acid 2-cyclohexyl-ethyl acrylamide (COA) / acrylic acid (AA)
Coming soon
1:1 ratio